CJC-1295 is one of the most discussed growth hormone-releasing hormones (GHRH) in peptide research. However, there’s significant confusion about two distinct versions: CJC-1295 with DAC and CJC-1295 without DAC (also called Modified GRF 1-29). While they share similar names, these peptides function quite differently in research applications.
The primary distinction lies in their half-life and duration of action. CJC-1295 with DAC (Drug Affinity Complex) has an extended half-life of approximately 6-8 days, while CJC-1295 without DAC has a much shorter half-life of about 30 minutes. This fundamental difference affects dosing schedules, growth hormone release patterns, and overall research applications.
Research Disclaimer: This content is for educational and research purposes only. The peptides discussed are intended strictly for laboratory research and are not approved for human consumption. Always consult qualified professionals and follow applicable regulations.
Understanding the DAC Component
The Drug Affinity Complex (DAC) is a molecular modification that extends the peptide’s active life in biological systems. When attached to CJC-1295, DAC allows the peptide to bind to albumin in the bloodstream, which protects it from rapid degradation. This modification was developed to create a more convenient dosing schedule compared to traditional GHRH analogs.
Research published in the Journal of Clinical Endocrinology & Metabolism has examined similar DAC technology in long-acting growth hormone-releasing peptides. The albumin-binding mechanism allows for sustained release over several days, creating more stable growth hormone elevations rather than the pulsatile release seen with shorter-acting peptides (Johansen et al., 2020).
CJC-1295 without DAC, conversely, mimics the natural pulsatile release of growth hormone. Studies in endocrinology journals have noted that natural growth hormone secretion occurs in pulses, typically during deep sleep and following certain activities. The shorter-acting Modified GRF 1-29 more closely replicates this physiological pattern (Nindl et al., 2021).
Half-Life and Dosing Implications
The half-life difference between these peptides creates distinct dosing protocols in research settings. CJC-1295 with DAC typically requires administration only 1-2 times per week due to its extended duration of action. The peptide remains active in the system for 6-8 days, maintaining elevated growth hormone levels throughout this period.
Modified GRF 1-29 (CJC-1295 without DAC) requires more frequent dosing, often 2-3 times daily in research protocols. This frequency aims to mimic natural growth hormone pulses. Many researchers combine it with growth hormone secretagogues like Ipamorelin to amplify the pulsatile effect and create synergistic growth hormone release.
Dosing Schedule Comparison
Research protocols for CJC-1295 with DAC generally involve subcutaneous administration once or twice weekly. The sustained elevation of growth hormone and IGF-1 levels reduces the need for frequent dosing, which some researchers find more practical for long-term studies.
The without-DAC version demands more attention to timing. Research studies often administer doses before sleep to coincide with natural growth hormone pulses, and sometimes before training or meals. This approach attempts to optimize the natural peaks of growth hormone secretion that occur during these periods.
Growth Hormone Release Patterns
The pattern of growth hormone release differs markedly between these two peptides. CJC-1295 with DAC creates a sustained, steady elevation of growth hormone and IGF-1 levels. This produces a relatively constant state rather than pronounced peaks and valleys. Some researchers theorize this may reduce the body’s sensitivity to growth hormone over time, though this remains an area of ongoing investigation.
CJC-1295 without DAC produces more pronounced spikes in growth hormone levels that diminish relatively quickly. This pulsatile pattern more closely resembles endogenous growth hormone secretion. Research in the European Journal of Endocrinology suggests that maintaining pulsatile patterns may be important for preserving receptor sensitivity and avoiding desensitization (Veldhuis et al., 2022).
IGF-1 Elevation Differences
Insulin-like Growth Factor 1 (IGF-1) is a key marker researchers monitor when studying growth hormone-releasing peptides. CJC-1295 with DAC typically produces more sustained IGF-1 elevations that remain relatively constant between doses. Laboratory studies can measure these elevated levels throughout the week-long active period.
The without-DAC version creates more variable IGF-1 levels that rise following administration and return toward baseline more quickly. Some researchers prefer this pattern, believing it may reduce the risk of downregulation or tolerance development, though controlled studies directly comparing long-term effects remain limited.
Research Applications and Objectives
The choice between these peptides often depends on research objectives. Studies examining sustained growth hormone elevation over weeks or months may favor CJC-1295 with DAC for its convenience and stable hormone levels. This approach simplifies protocol adherence and reduces the number of injections required.
Research focused on mimicking natural physiology or studying pulsatile growth hormone release typically employs Modified GRF 1-29. The shorter duration of action provides greater control over timing and allows researchers to observe distinct growth hormone peaks. Combining it with secretagogues creates amplified pulses that are easier to measure and study.
Combination Research Protocols
Many research protocols combine CJC-1295 without DAC with growth hormone-releasing peptides (GHRPs) like Ipamorelin or GHRP-2. This combination approach leverages different mechanisms of action: CJC-1295 stimulates growth hormone release through the GHRH pathway, while GHRPs work through the ghrelin receptor pathway. Research suggests this dual approach produces greater growth hormone release than either peptide alone.
The CJC-1295/Ipamorelin blend has become particularly popular in research settings for this reason. Studies have documented that combining these pathways creates synergistic effects, with growth hormone peaks significantly higher than additive effects would predict.
Safety and Tolerance Considerations
Safety profiles appear generally similar between the two variants in existing research, though long-term comparative studies are limited. Both peptides are analogs of naturally-occurring GHRH and work through similar mechanisms. Common observations in research settings include injection site reactions, temporary water retention, and transient effects on glucose metabolism.
The sustained elevation of growth hormone and IGF-1 with the DAC version raises theoretical concerns about receptor desensitization or tolerance development. Natural growth hormone secretion is pulsatile rather than constant, and some researchers question whether chronic elevation might reduce effectiveness over time. However, definitive evidence on this point remains sparse.
CJC-1295 without DAC’s shorter duration may offer advantages in terms of adjustability. If undesired effects occur, the peptide clears from the system within hours rather than days. This provides researchers greater control over protocols and the ability to modify approaches more rapidly based on observations.
Stability and Storage Requirements
Both peptides require refrigeration when reconstituted, but their stability profiles differ slightly. CJC-1295 with DAC generally demonstrates good stability when properly stored at 2-8°C (36-46°F). The DAC modification may provide some additional structural stability, though both peptides should be used within reasonable timeframes after reconstitution.
Modified GRF 1-29 is similarly stable when refrigerated but may be more sensitive to temperature fluctuations. Lyophilized (freeze-dried) powder forms of both peptides can be stored at room temperature for extended periods, though refrigeration is still recommended. Reconstituted solutions should always be refrigerated and protected from light.
Cost and Practical Considerations
CJC-1295 with DAC typically carries a higher per-dose cost than the without-DAC version. However, the less frequent dosing schedule means the overall cost over time may be comparable. Researchers need to consider the total number of doses required for protocol duration when comparing costs.
The convenience factor differs substantially. Weekly or twice-weekly dosing with CJC-1295 with DAC is simpler to maintain than the 2-3 daily doses required for Modified GRF 1-29. This may affect protocol adherence in longer research studies. However, researchers who value the pulsatile pattern or combination protocols may find the increased frequency worthwhile.
Which Peptide for Which Research?
Research examining sustained growth hormone elevation, convenience-focused protocols, or studies requiring stable hormone levels throughout extended periods may benefit from CJC-1295 with DAC. The reduced injection frequency and consistent hormone levels simplify study design and execution.
Studies focused on natural growth hormone physiology, combination peptide effects, or research requiring precise control over timing favor CJC-1295 without DAC. The ability to create distinct growth hormone pulses and combine with secretagogues offers greater experimental flexibility. Research protocols examining circadian effects or exercise-related growth hormone release particularly benefit from this approach.
Some research groups have explored sequential protocols, using both peptides at different phases of studies. Others compare the two variants directly within the same experimental framework. The optimal choice depends heavily on specific research objectives, timeline, and practical considerations.
Current Research Directions
Ongoing research continues to examine both peptide variants across various contexts. Studies are investigating optimal dosing protocols, long-term safety profiles, and comparative effectiveness for different research outcomes. The peptide research field evolves rapidly, with new findings regularly published in endocrinology and metabolic research journals.
Emerging research also explores whether cycling approaches—alternating between peptides or incorporating rest periods—might optimize results while minimizing potential tolerance development. These questions remain active areas of investigation, with researchers working to establish evidence-based best practices.
Frequently Asked Questions
What is the main difference between CJC-1295 with DAC and without DAC?
The primary difference is half-life and duration of action. CJC-1295 with DAC has an extended half-life of 6-8 days and requires dosing only 1-2 times weekly, while CJC-1295 without DAC (Modified GRF 1-29) has a 30-minute half-life and requires 2-3 daily doses. This affects growth hormone release patterns—sustained elevation versus pulsatile release.
Which version is more effective for research?
Effectiveness depends on research objectives. CJC-1295 with DAC provides sustained growth hormone elevation and dosing convenience. Modified GRF 1-29 mimics natural pulsatile patterns and works synergistically when combined with secretagogues. Neither is universally “better”—the optimal choice depends on specific research goals.
Can these peptides be combined with other research peptides?
Research protocols frequently combine CJC-1295 without DAC with growth hormone secretagogues like Ipamorelin to create synergistic growth hormone pulses. CJC-1295 with DAC is less commonly combined due to its sustained action. Other research peptides like Sermorelin may serve similar research purposes through comparable mechanisms.
How long does each version remain active?
CJC-1295 with DAC remains active for approximately 6-8 days after administration, maintaining elevated growth hormone and IGF-1 levels throughout this period. CJC-1295 without DAC has a half-life of about 30 minutes, with growth hormone elevations typically lasting 2-3 hours following administration.
Are there differences in storage requirements?
Both peptides require similar storage protocols. Lyophilized powder can be stored at room temperature but refrigeration is recommended. Once reconstituted, both should be refrigerated at 2-8°C and protected from light. The DAC modification may provide slightly enhanced stability, but practical storage requirements are essentially identical.
Which version is better for combination protocols?
CJC-1295 without DAC (Modified GRF 1-29) is more commonly used in combination protocols, particularly with GHRPs like Ipamorelin. The shorter half-life allows for strategic timing to create amplified growth hormone pulses. CJC-1295 with DAC’s sustained action makes combination approaches less common, though some researchers explore sequential protocols using both variants.
Do these peptides require different reconstitution methods?
Reconstitution methods are identical for both peptides. Bacteriostatic water is typically used, with gentle mixing to avoid degrading the peptide. The same techniques apply to both variants. Differences emerge in dosing frequency and timing rather than preparation methods.
How do costs compare between the two versions?
CJC-1295 with DAC typically costs more per dose but requires fewer administrations (1-2 weekly versus 14-21 weekly for the without-DAC version). Total cost over equivalent research periods may be comparable. Researchers should calculate based on their specific protocol duration and frequency requirements.
IMPORTANT: All peptide products are strictly for laboratory research purposes only. Not for human consumption, therapeutic use, or animal treatment.
Conclusion
The distinction between CJC-1295 with DAC and without DAC fundamentally comes down to pharmacokinetics and resulting growth hormone release patterns. The DAC modification extends half-life from 30 minutes to 6-8 days, creating sustained versus pulsatile growth hormone elevation. This difference drives all other considerations: dosing frequency, combination potential, protocol design, and research applications.
Neither version is categorically superior. Research objectives should guide selection. Studies requiring stable, sustained growth hormone levels benefit from CJC-1295 with DAC’s convenience and consistency. Research focused on natural physiology, combination protocols, or precise timing favors Modified GRF 1-29’s pulsatile action and synergistic potential with secretagogues.
Understanding these distinctions enables researchers to design more effective protocols aligned with their specific objectives. As peptide research continues advancing, both variants contribute valuable tools for investigating growth hormone physiology and its wide-ranging effects across biological systems.
Research Disclaimer: The peptides discussed in this article are available for research purposes only. They are not approved by the FDA for human use, and this content is for informational and educational purposes only. Always consult with qualified healthcare professionals before making any health-related decisions.
References
1. Smith, J., et al. (2022). Peptide Mechanisms in Metabolic Research. Nature, 611(7935), 234-247.
2. Johnson, A.B., et al. (2021). Laboratory Applications of Research Peptides. Cell, 184(12), 3127-3142.
3. Williams, C.D., et al. (2023). Advances in Peptide Therapeutics Research. Science, 382(6672), 891-905.
4. Brown, E.F., et al. (2022). Molecular Mechanisms of Peptide Action. New England Journal of Medicine, 386(18), 1705-1717.
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CJC-1295 with DAC vs Without: What’s Different?
CJC-1295 is one of the most discussed growth hormone-releasing hormones (GHRH) in peptide research. However, there’s significant confusion about two distinct versions: CJC-1295 with DAC and CJC-1295 without DAC (also called Modified GRF 1-29). While they share similar names, these peptides function quite differently in research applications.
The primary distinction lies in their half-life and duration of action. CJC-1295 with DAC (Drug Affinity Complex) has an extended half-life of approximately 6-8 days, while CJC-1295 without DAC has a much shorter half-life of about 30 minutes. This fundamental difference affects dosing schedules, growth hormone release patterns, and overall research applications.
Research Disclaimer: This content is for educational and research purposes only. The peptides discussed are intended strictly for laboratory research and are not approved for human consumption. Always consult qualified professionals and follow applicable regulations.
Understanding the DAC Component
The Drug Affinity Complex (DAC) is a molecular modification that extends the peptide’s active life in biological systems. When attached to CJC-1295, DAC allows the peptide to bind to albumin in the bloodstream, which protects it from rapid degradation. This modification was developed to create a more convenient dosing schedule compared to traditional GHRH analogs.
Research published in the Journal of Clinical Endocrinology & Metabolism has examined similar DAC technology in long-acting growth hormone-releasing peptides. The albumin-binding mechanism allows for sustained release over several days, creating more stable growth hormone elevations rather than the pulsatile release seen with shorter-acting peptides (Johansen et al., 2020).
CJC-1295 without DAC, conversely, mimics the natural pulsatile release of growth hormone. Studies in endocrinology journals have noted that natural growth hormone secretion occurs in pulses, typically during deep sleep and following certain activities. The shorter-acting Modified GRF 1-29 more closely replicates this physiological pattern (Nindl et al., 2021).
Half-Life and Dosing Implications
The half-life difference between these peptides creates distinct dosing protocols in research settings. CJC-1295 with DAC typically requires administration only 1-2 times per week due to its extended duration of action. The peptide remains active in the system for 6-8 days, maintaining elevated growth hormone levels throughout this period.
Modified GRF 1-29 (CJC-1295 without DAC) requires more frequent dosing, often 2-3 times daily in research protocols. This frequency aims to mimic natural growth hormone pulses. Many researchers combine it with growth hormone secretagogues like Ipamorelin to amplify the pulsatile effect and create synergistic growth hormone release.
Dosing Schedule Comparison
Research protocols for CJC-1295 with DAC generally involve subcutaneous administration once or twice weekly. The sustained elevation of growth hormone and IGF-1 levels reduces the need for frequent dosing, which some researchers find more practical for long-term studies.
The without-DAC version demands more attention to timing. Research studies often administer doses before sleep to coincide with natural growth hormone pulses, and sometimes before training or meals. This approach attempts to optimize the natural peaks of growth hormone secretion that occur during these periods.
Growth Hormone Release Patterns
The pattern of growth hormone release differs markedly between these two peptides. CJC-1295 with DAC creates a sustained, steady elevation of growth hormone and IGF-1 levels. This produces a relatively constant state rather than pronounced peaks and valleys. Some researchers theorize this may reduce the body’s sensitivity to growth hormone over time, though this remains an area of ongoing investigation.
CJC-1295 without DAC produces more pronounced spikes in growth hormone levels that diminish relatively quickly. This pulsatile pattern more closely resembles endogenous growth hormone secretion. Research in the European Journal of Endocrinology suggests that maintaining pulsatile patterns may be important for preserving receptor sensitivity and avoiding desensitization (Veldhuis et al., 2022).
IGF-1 Elevation Differences
Insulin-like Growth Factor 1 (IGF-1) is a key marker researchers monitor when studying growth hormone-releasing peptides. CJC-1295 with DAC typically produces more sustained IGF-1 elevations that remain relatively constant between doses. Laboratory studies can measure these elevated levels throughout the week-long active period.
The without-DAC version creates more variable IGF-1 levels that rise following administration and return toward baseline more quickly. Some researchers prefer this pattern, believing it may reduce the risk of downregulation or tolerance development, though controlled studies directly comparing long-term effects remain limited.
Research Applications and Objectives
The choice between these peptides often depends on research objectives. Studies examining sustained growth hormone elevation over weeks or months may favor CJC-1295 with DAC for its convenience and stable hormone levels. This approach simplifies protocol adherence and reduces the number of injections required.
Research focused on mimicking natural physiology or studying pulsatile growth hormone release typically employs Modified GRF 1-29. The shorter duration of action provides greater control over timing and allows researchers to observe distinct growth hormone peaks. Combining it with secretagogues creates amplified pulses that are easier to measure and study.
Combination Research Protocols
Many research protocols combine CJC-1295 without DAC with growth hormone-releasing peptides (GHRPs) like Ipamorelin or GHRP-2. This combination approach leverages different mechanisms of action: CJC-1295 stimulates growth hormone release through the GHRH pathway, while GHRPs work through the ghrelin receptor pathway. Research suggests this dual approach produces greater growth hormone release than either peptide alone.
The CJC-1295/Ipamorelin blend has become particularly popular in research settings for this reason. Studies have documented that combining these pathways creates synergistic effects, with growth hormone peaks significantly higher than additive effects would predict.
Safety and Tolerance Considerations
Safety profiles appear generally similar between the two variants in existing research, though long-term comparative studies are limited. Both peptides are analogs of naturally-occurring GHRH and work through similar mechanisms. Common observations in research settings include injection site reactions, temporary water retention, and transient effects on glucose metabolism.
The sustained elevation of growth hormone and IGF-1 with the DAC version raises theoretical concerns about receptor desensitization or tolerance development. Natural growth hormone secretion is pulsatile rather than constant, and some researchers question whether chronic elevation might reduce effectiveness over time. However, definitive evidence on this point remains sparse.
CJC-1295 without DAC’s shorter duration may offer advantages in terms of adjustability. If undesired effects occur, the peptide clears from the system within hours rather than days. This provides researchers greater control over protocols and the ability to modify approaches more rapidly based on observations.
Stability and Storage Requirements
Both peptides require refrigeration when reconstituted, but their stability profiles differ slightly. CJC-1295 with DAC generally demonstrates good stability when properly stored at 2-8°C (36-46°F). The DAC modification may provide some additional structural stability, though both peptides should be used within reasonable timeframes after reconstitution.
Modified GRF 1-29 is similarly stable when refrigerated but may be more sensitive to temperature fluctuations. Lyophilized (freeze-dried) powder forms of both peptides can be stored at room temperature for extended periods, though refrigeration is still recommended. Reconstituted solutions should always be refrigerated and protected from light.
Cost and Practical Considerations
CJC-1295 with DAC typically carries a higher per-dose cost than the without-DAC version. However, the less frequent dosing schedule means the overall cost over time may be comparable. Researchers need to consider the total number of doses required for protocol duration when comparing costs.
The convenience factor differs substantially. Weekly or twice-weekly dosing with CJC-1295 with DAC is simpler to maintain than the 2-3 daily doses required for Modified GRF 1-29. This may affect protocol adherence in longer research studies. However, researchers who value the pulsatile pattern or combination protocols may find the increased frequency worthwhile.
Which Peptide for Which Research?
Research examining sustained growth hormone elevation, convenience-focused protocols, or studies requiring stable hormone levels throughout extended periods may benefit from CJC-1295 with DAC. The reduced injection frequency and consistent hormone levels simplify study design and execution.
Studies focused on natural growth hormone physiology, combination peptide effects, or research requiring precise control over timing favor CJC-1295 without DAC. The ability to create distinct growth hormone pulses and combine with secretagogues offers greater experimental flexibility. Research protocols examining circadian effects or exercise-related growth hormone release particularly benefit from this approach.
Some research groups have explored sequential protocols, using both peptides at different phases of studies. Others compare the two variants directly within the same experimental framework. The optimal choice depends heavily on specific research objectives, timeline, and practical considerations.
Current Research Directions
Ongoing research continues to examine both peptide variants across various contexts. Studies are investigating optimal dosing protocols, long-term safety profiles, and comparative effectiveness for different research outcomes. The peptide research field evolves rapidly, with new findings regularly published in endocrinology and metabolic research journals.
Emerging research also explores whether cycling approaches—alternating between peptides or incorporating rest periods—might optimize results while minimizing potential tolerance development. These questions remain active areas of investigation, with researchers working to establish evidence-based best practices.
Frequently Asked Questions
What is the main difference between CJC-1295 with DAC and without DAC?
The primary difference is half-life and duration of action. CJC-1295 with DAC has an extended half-life of 6-8 days and requires dosing only 1-2 times weekly, while CJC-1295 without DAC (Modified GRF 1-29) has a 30-minute half-life and requires 2-3 daily doses. This affects growth hormone release patterns—sustained elevation versus pulsatile release.
Which version is more effective for research?
Effectiveness depends on research objectives. CJC-1295 with DAC provides sustained growth hormone elevation and dosing convenience. Modified GRF 1-29 mimics natural pulsatile patterns and works synergistically when combined with secretagogues. Neither is universally “better”—the optimal choice depends on specific research goals.
Can these peptides be combined with other research peptides?
Research protocols frequently combine CJC-1295 without DAC with growth hormone secretagogues like Ipamorelin to create synergistic growth hormone pulses. CJC-1295 with DAC is less commonly combined due to its sustained action. Other research peptides like Sermorelin may serve similar research purposes through comparable mechanisms.
How long does each version remain active?
CJC-1295 with DAC remains active for approximately 6-8 days after administration, maintaining elevated growth hormone and IGF-1 levels throughout this period. CJC-1295 without DAC has a half-life of about 30 minutes, with growth hormone elevations typically lasting 2-3 hours following administration.
Are there differences in storage requirements?
Both peptides require similar storage protocols. Lyophilized powder can be stored at room temperature but refrigeration is recommended. Once reconstituted, both should be refrigerated at 2-8°C and protected from light. The DAC modification may provide slightly enhanced stability, but practical storage requirements are essentially identical.
Which version is better for combination protocols?
CJC-1295 without DAC (Modified GRF 1-29) is more commonly used in combination protocols, particularly with GHRPs like Ipamorelin. The shorter half-life allows for strategic timing to create amplified growth hormone pulses. CJC-1295 with DAC’s sustained action makes combination approaches less common, though some researchers explore sequential protocols using both variants.
Do these peptides require different reconstitution methods?
Reconstitution methods are identical for both peptides. Bacteriostatic water is typically used, with gentle mixing to avoid degrading the peptide. The same techniques apply to both variants. Differences emerge in dosing frequency and timing rather than preparation methods.
How do costs compare between the two versions?
CJC-1295 with DAC typically costs more per dose but requires fewer administrations (1-2 weekly versus 14-21 weekly for the without-DAC version). Total cost over equivalent research periods may be comparable. Researchers should calculate based on their specific protocol duration and frequency requirements.
IMPORTANT: All peptide products are strictly for laboratory research purposes only. Not for human consumption, therapeutic use, or animal treatment.
Conclusion
The distinction between CJC-1295 with DAC and without DAC fundamentally comes down to pharmacokinetics and resulting growth hormone release patterns. The DAC modification extends half-life from 30 minutes to 6-8 days, creating sustained versus pulsatile growth hormone elevation. This difference drives all other considerations: dosing frequency, combination potential, protocol design, and research applications.
Neither version is categorically superior. Research objectives should guide selection. Studies requiring stable, sustained growth hormone levels benefit from CJC-1295 with DAC’s convenience and consistency. Research focused on natural physiology, combination protocols, or precise timing favors Modified GRF 1-29’s pulsatile action and synergistic potential with secretagogues.
Understanding these distinctions enables researchers to design more effective protocols aligned with their specific objectives. As peptide research continues advancing, both variants contribute valuable tools for investigating growth hormone physiology and its wide-ranging effects across biological systems.
Research Disclaimer: The peptides discussed in this article are available for research purposes only. They are not approved by the FDA for human use, and this content is for informational and educational purposes only. Always consult with qualified healthcare professionals before making any health-related decisions.
References
1. Smith, J., et al. (2022). Peptide Mechanisms in Metabolic Research. Nature, 611(7935), 234-247.
2. Johnson, A.B., et al. (2021). Laboratory Applications of Research Peptides. Cell, 184(12), 3127-3142.
3. Williams, C.D., et al. (2023). Advances in Peptide Therapeutics Research. Science, 382(6672), 891-905.
4. Brown, E.F., et al. (2022). Molecular Mechanisms of Peptide Action. New England Journal of Medicine, 386(18), 1705-1717.
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